Bio-Rad ChromLab™ Software - Manual (Page 141)

Preparing the System and Sample Pump Piston Washing Systems
Instrument Guide | 139
Preparing the System and Sample Pump Piston Washing
Systems
Note: Place the washing systems reservoir for the system and sample pumps
at the same height or above the level of the pump heads to avoid siphoning of
the solution back to the reservoir.
If the washing system is not thoroughly primed and devoid of air, recirculation might
not occur at flow rates below
 0.3 ml/min for the F10 pump piston washing system
 3.0 ml/min for the F100 pump piston washing system
If routinely operating at low flow rates, reprime the washing system to ensure
recirculation and efficient piston washing. You can reprime the system pumps by
operating the pump for a brief period at a flow rate of
 1.0 ml/min or greater for the F10 pump piston washing system
 10 ml/min or greater for the F100 pump piston washing system
Tip: You can also reprime the system manually as explained in the section
Priming and Purging the Systems on page 118 .