Bio-Rad ChromLab™ Software User Manual
Page 33

Pumps
Instrument Guide
| 31
Each system pump module has an emergency Pause button and a Purge button.
Pressing the emergency Pause button on either system pump stops
both
pumps. If a run is in progress when you press Pause on either system
pump, both system pumps stop and the run pauses.
You can resume or cancel the run through ChromLab. If you choose to cancel
the run, you can save the run data up to that point.
Pressing Purge on a system pump runs that pump at full speed, replacing
any air in the lines with buffer. Purge activates purging for the tubing lines
that belong to that specific pump. For instance, when you press Purge on
system pump A, pump A runs at maximum flow rate while pump B
becomes idle. If you press Purge on pump B while pump A is purging, the
purge on pump A stops and the purge on pump B starts. The Purge button
is a toggle. To stop the purge, press Purge on that system pump again.
Note:
The Purge button is deactivated when:
A run is in progress
Any of the modules are in calibration mode
The buffer blending valve is included in the fluidic scheme
Caution:
To avoid damaging the column, always ensure that it is offline
when purging the system. Set the inject valve to waste, or remove the
column from the system and replace with a union fitting.
Tip:
You can pause the system through ChromLab using either the touch
screen or the computer running ChromLab (known in this document as the
ChromLab computer). You can also set timed purging of both pumps through
ChromLab, which will set the inject valve to Waste for a set amount of time. For
more information, see the
NGC Chromatography Systems and ChromLab
Software User Guide.
Pump Priming Port
The pump priming port introduces fluid into, and removes air from, the inlet tubing
line and the pump head.