Bio-Rad ChromLab™ Software - Manual (Page 32)

2 | The NGC Instrument
30 | NGC Chromatography Systems and ChromLab Software
Detailed Attributes
The NGC systems include two system pumps: pump A (the left pump) and pump B
(the right pump). The system pumps are available in two flow rates: 10 ml/min (the
F10 pumps) and 100 ml/min (the F100 pumps).
F10 Pump
 Flow rate delivery: 0.001–10 ml/min specified
 Operating pressure range: 0–3,650 psi (0–25.2 MPa)
 Operating viscosity range: 0.35–10 cP
F100 Pump
 Flow rate delivery: 0.01–100 ml/min specified
 Operating pressure range: 0–1,450 psi (0–10 MPa)
 Operating viscosity range: 0.35–10 cP
System Pump LEDs
Each system pump module is fitted with LEDs.
 Green LEDs indicate the pump is in use.
How the System Pumps Function
Each NGC system ships with either two F10 pumps or two F100 pumps. The
reciprocating pistons in each pump synchronize to deliver continuous flow with low
pulsation. On the NGC Quest system (without a buffer blending valve), the system
pumps operate such that the sum of the flow rates of pump A and pump B is equal
to the maximum delivery rate. For example, if the flow of an F10 pump A equals 10
ml/min, then the rate for the F10 pump B must equal 0 ml/min. If the system is set to
deliver 5 ml/min with a gradient mixture of 50% B, pump A and pump B each run at
2.5 ml/min.