Bio-Rad ChromLab™ Software - Manual (Page 253)

System and Sample Pumps
Instrument Guide | 251
Backpressure values
are higher or lower than
expected. The flow rate might have changed. In ChromLab, verify that the
pump is set to deliver the
correct flow rate.
Note: Recalibrate after
stopping the pumps. In
ChromLab, select Tools >
Calibrate > Pump Flow
Rate. Complete the
calibration steps in the
dialog box.
The column might need to be
cleaned, or a frit might need to be
replaced.  Remove the column and run
the pumps to determine if the
high backpressure is due to
the column.
 Consult the column’s user
manual for the cleaning
procedure.
Note: Recalibrate after
stopping the pumps. In
ChromLab, select Tools >
Calibrate > Pump Flow
Rate. Complete the
calibration steps in the
dialog box.
The pressure transducer
calibration might be incorrect.  Inspect the tubing path for
obstructions.
 Change the tubing in the
tubing path if necessary.
Note: Recalibrate after
stopping the pumps. In
ChromLab, select Tools >
Calibrate > Pump Flow
Rate. Complete the
calibration steps in the
dialog box.
Symptom Possible Cause Possible Solution