Priming and purging the ngc scout system, Priming the ngc scout system – Bio-Rad ChromLab™ Software User Manual
Page 124
3
| Preparing the Instrument
122
| NGC Chromatography Systems and ChromLab Software
Priming and Purging the NGC Scout System
Priming the NGC Scout system is a manual procedure and involves priming the
buffer blending valve starting from the Q4 port to Q1. You can purge one line at a
time, or all lines sequentially. These procedures require access to ChromLab.
Tip:
You can perform the steps on the NGC touch screen.
Priming the NGC Scout System
Note:
This section assumes that you have not introduced buffer into the lines.
If you have buffer in the lines and are purging the lines of small air bubbles,
proceed to
Purging the NGC Scout System on page
.
To prime the buffer blending valve
1.
Immerse the inlet tubing for ports Q1–Q4 in their respective buffer containers.
(See
To plumb the buffer blending valve on page
for more information.)
2.
On the ChromLab computer or the touch screen, start ChromLab in Manual
mode.
3.
Set the fluidic scheme to either NGC Scout or NGC Scout Plus.
4.
In the fluidic scheme, touch or click the System Pump module to open its
dialog box.