Bio-Rad ChromLab™ Software - Manual (Page 187)

System and Sample Pumps
Instrument Guide | 185
Conditioning New Seals
New seals should be conditioned before use. To condition the seals, run the pump
with wetted seals under controlled flow rate and pressure conditions. This allows
the seals to seat properly and proof against leaks.
Important: Use only organic solvents to condition new seals. Buffer solutions
and salt solutions should never be used to condition new seals. Recommended
solvents are HPLC-grade methanol and IPA or aqueous mixtures of these
solvents.
To condition the seals
1. Using a backpressure regulator or a suitable column, run the pump with a 50:50
solution of IPA (or methanol) and water for 30 min at the backpressure and flow
rate listed in Table
16 for the pump head type.
2. At the end of the 30-min treatment, run the pump for an additional 15 min at the
backpressure and flow rate listed in Table
17.
Table 16. Initial backpressure and flow rates for conditioning seals
Pump Type Backpressure Flow Rate
10 ml/min 2,000 psi 2 ml/min
100 ml/min 1,000 psi 2 ml/min
Table 17. Secondary backpressure and flow rates for conditioning seals
Pump Type Backpressure Flow Rate
10 ml/min 3,250 psi 4 ml/min
100 ml/min 1,300 psi 5 ml/min