Bio-Rad ChromLab™ Software - Manual (Page 145)

Preparing the Columns
Instrument Guide | 143
Washing the Columns
Cleaning a column requires access to ChromLab. You can perform this task from
the ChromLab computer or the NGC touch screen. This procedure explains how to
manually wash columns through the System Control tab in ChromLab. Alternatively,
you can wash columns in the following phases in the Method Editor: System
Preparation, Column Activation, or Column Wash. See the NGC Chromatography
Systems and ChromLab Software User Guide for more information about these
phases.
Caution: If you wash the column in the reverse flow direction, set the flow rate
to half the column’s recommended flow rate (keep the pressure below the
recommended maximum).
Important: Verify compatibility before using a wash solvent. Many wash
solvents are a higher viscosity than the typical eluting solvents. You might need
to reduce the flow rate to avoid exceeding the maximum allowable pressure.
Refer to the column specification sheet for recommended cleaning solutions,
flow rates, column volume rates, and storage solutions.
To wash a column
1. Immerse the system pump inlet line in the buffer solution.
2. In the fluidic scheme, touch or click the System Pump module to open its
dialog box.
3. In the System Pump dialog box:
a. For the NGC Scout and NGC Discover systems, on the Mode dropdown
list select one of the following:
 Gradient via Inlet Valves
 Gradient via Blending Valve (High Flow)
b. Set Flow Rate to 1 ml/min (or manufacturer’s specification).
c. Set Mode to Isocratic.
d. Set Duration to 3–5 min (or manufacturer’s specification).