Bio-Rad ChromLab™ Software - Manual (Page 147)

Calibrating the NGC Instrument
Instrument Guide | 145
Calibrating the NGC Instrument
The NGC systems include integrated pressure and temperature sensors. The
pressure sensors monitor the system pressure to protect the column and media
from overpressure. The temperature sensor in the conductivity monitor enables the
system to provide real-time temperature compensation for conductivity and pH
measurements. The measured temperature appears as a trace on the
chromatogram.
All sensors and detectors on the NGC systems ship precalibrated to ensure
accurate monitoring. ChromLab enables you to recalibrate the pump flow rate and
the conductivity and the pH monitors, zero the pressure sensors, and reset them to
their factory settings.
Note: You cannot run a method while calibrating the instrument.
Tip: This section provides general procedures for calibrating the modules. For
more information about using ChromLab to calibrate the modules, see the
NGC
Chromatography Systems and ChromLab Software User Guide.
Calibrating pH
Use two pH calibration buffers with a difference of at least one pH unit. Allow the
buffers to reach the operating temperature before use.
To calibrate pH
1. Do one of the following:
 On the ChromLab computer, select the System Control tab, click
Tools > Calibrate, and select pH on the dropdown list.
 On the NGC touch screen, select Calibrate on the ChromLab dropdown
menu and then select pH on the dropdown list.
2. Follow the steps in the pH Calibration dialog box.
3. Verify that Status (at the bottom of the dialog box) is Calibrated.