Bio-Rad ChromLab™ Software - Manual (Page 100)

3 | Preparing the Instrument
98 | NGC Chromatography Systems and ChromLab Software
Changing the Mixer Barrel
The mixer module is used to blend buffers from two to four separate solutions. It is
important to use a mixer with a barrel size that is based on the flow rate of
separation. Bio-Rad offers several different mixer barrel sizes for different flow rates.
The F10 systems ship with the 263 μ l mixer base and top assembly. When using a
higher flow rate, the mixer volume of this base unit can be expanded by adding a
750 μl or 2 ml barrel. The F100 systems ship with 750 μl mixer base and top
assembly. The mixer volume can be increased at higher flow rates by inserting
either 2ml, 5 ml, or 12 ml barrel.
Table 10 lists the recommended barrel size for different flow rates for systems
without a buffer blending valve (gradient mode).
Table 11 on page 99 lists the recommended barrel size for different flow rates for
systems with a buffer blending valve.
Tip: For liquids that are difficult to mix, use a mixer barrel with a higher
capacity.
Table 10. Recommended NGC system mixer barrel sizes —
without buffer blending valve (gradient)
Barrel Size Flow Rate, ml/min
F10 Pump F100 Pump
263 μl
(Base and top)
0.1–1.00 n/a
750 μl
(Base and top)
1.00–9.99 1.00–4.99
2 ml 10.00–20.00 5.00–14.99
5 ml n/a 15.00–39.99
12 ml n/a 40.00–200.00