Bio-Rad ChromLab™ Software - Manual (Page 125)

Priming and Purging the Systems
Instrument Guide | 123
5. In the System Pump dialog box, select Priming on the Mode dropdown list.
Note: When in Priming mode, the sample inject valve switches the system
pump flow to waste (W2). After the process is completed, the sample inject
valve switches the system pump to manually load the column through the
loop.
By default, all ports are closed. The Closed radio button is selected.
6. Select Q4 as the port to open and prime.
7. Insert a 30 ml syringe into the priming port on system pump A.
8. Turn the priming port valve counter-clockwise one full turn to open the seal.
9. Slowly withdraw the plunger to withdraw liquid from the port until air no
longer enters the syringe (approximately 10 ml) and the tubing is filled with
liquid.
10. Turn the priming port valve clockwise to close the port.
11. Remove the syringe from the priming port and discard the liquid into waste.
12. Repeat steps 7–11 for ports Q3–Q1.
13. To prime system pump B, select port Q3 perform steps 7–11.