Bio-Rad EXQuest Spot Cutter User Manual
Page 530
Appendix L. Techniques
L-5
Fig. L-3. Gel Record Editor.
Enter the date on which the gel was run in the text box next to the Gel run date
prompt.
Next to the Calstrip prompt, click on the button that corresponds to the kind of
calibration associated with this gel. In most cases, you or someone in your lab has
made the calstrips, so the button labeled User Batch is the appropriate choice.
Next to the User Batch prompt, click on the arrow. From the list displayed, select the
calibration record name for the calstrip. The Detection and Isotope fields are
automatically obtained from the specified Calstrip Record.
Click on the appropriate button to indicate the type of detection used for your gel, if
not already defined from the batch file.
Click on the Isotope button to indicate the isotope used in the gel. This is usually,
though not necessarily, the same isotope as the one used in the calstrip.
Next, enter the number of counts loaded on the gel.
Enter the day on which you began exposing the gel to film in the text box next to the
Exposure start prompt.