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E.5 calibration – Bio-Rad EXQuest Spot Cutter User Manual

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Appendix E. GS-710

E-9

Specifying Your Own Resolution

If you select Oversample under More Options, you can specify your own resolution
within the range of 43–169 microns (micrometers). With Oversample selected, enter
values directly in the fields next to X resolution and Y resolution in the main
acquisition window.

Fig. E-8. Entering a custom resolution (with Oversample selected).

Image File Size

Image File Size (under Select Resolution) shows the size of the scan file you are
about to create. If you do not have enough computer memory for the specified file
size, an error message will appear when you attempt to scan. If this happens, select a
lower resolution or decrease the size of the area to be scanned. (Macintosh users can
also increase the application memory partition. See your Macintosh computer
documentation for guidance.)

E.5 Calibration

Images scanned using the GS-710 are automatically calibrated. The scanner plate has
a built-in calibration overlay for both transmissive and reflective scanning.

The transmissive calibration strip calibrates the densitometer from 0 to 3.0 O.D.

The reflective calibration strip calibrates the densitometer to 2.0 O.D.

The first time you use the GS-710, you must select some calibration settings to ensure
that your calibration is accurate.

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