Bio-Rad EXQuest Spot Cutter User Manual
Page 310
Integrated Excision Tool
9-21
Note:
You always have the option of manually correcting spot positions.
Auto-alignment is not always correct and the manual collection allows you to
readjust your spot positions.
Flag the spot you want to correct in the cutter camera image and drag the spot to the
correct position. Red indicates the spot is below the confidence threshold, green spots
indicate the spot is above the confidence threshold, and the spot becomes white while
it is dragged.
You also have the option of returning to Data Box Positioning.
Step 1 Light Options
To acquire this image, first select the light option appropriate for your gel/membrane/
stain type:
•
Light on (gels).
•
Light off (PVDF membranes). Ambient light will produce a better image for
PVDF membranes.
•
UV on (fluorescent gels). UV light is only available for the Fluorescent Spot
Cutters.
Fig. 9-10. Light settings.
If UV light is selected, specify an exposure time in seconds in the field. Also, make
sure that the UV lamp switch on the back of the cutter box is on.
Note:
For Sypro Ruby stains, an exposure time of 20-30 seconds is recommended.
When you have selected a light option, click Acquire Image.