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PDQuest User Guide
iv
3.4. Tiling Windows ............................................................................................... 3-4
3.5. Magnifying Images ......................................................................................... 3-4
3.6. Positioning Images .......................................................................................... 3-7
3.7. Hiding Overlays ............................................................................................ 3-10
3.8. Density Tools ................................................................................................ 3-11
3.9. Colors ............................................................................................................ 3-13
3.10. Multi-Channel Viewer ................................................................................ 3-16
3.11. 3D Viewer ................................................................................................... 3-18
3.12. Transform .................................................................................................... 3-20
3.13. Cropping Images ......................................................................................... 3-26
3.14. Flipping and Rotating Images ..................................................................... 3-29
3.15. Filtering Images .......................................................................................... 3-32
3.16. Invert Data ................................................................................................... 3-38
3.17. Text Overlays .............................................................................................. 3-38
4.
Detecting and Editing Spots ............................................. 4-1
4.1. Selecting Spot Detection Parameters .............................................................. 4-1
4.2. Detecting Spots .............................................................................................. 4-9
4.3. Filtered and Gaussian Images ....................................................................... 4-12
4.4. Spot Crosshairs and Ellipses ......................................................................... 4-14
4.5. Adding and Removing Spots ........................................................................ 4-15
4.6. Spot Boundary Tools .................................................................................... 4-18
4.7. Cancelling Spots ........................................................................................... 4-22
4.8. Combining Spots ........................................................................................... 4-23
4.9. Saturated and Faint Spots .............................................................................. 4-23
4.10. Spot Quantity .............................................................................................. 4-24
4.11. Spot Quality ................................................................................................ 4-26
4.12. Spot Parameters ........................................................................................... 4-28
4.13. Finding Spots .............................................................................................. 4-28
5.
MatchSets ........................................................................... 5-1
5.1. Creating a MatchSet ........................................................................................ 5-3
