Bio-Rad EXQuest Spot Cutter - Manual (Page 457)

PDQuest User Guide
G-10
A preview scan takes only half as long to create as a real scan, because the preview
scan does not capture a “dark” image (see the following section on Options). The
progress of the exposure will be displayed in the Exposure Status bar at the bottom of
the dialog box.
You cannot save preview scans.
If you want to stop a preview scan that is in progress, click on the Stop button.
G.5 Acquire the Image
The Fluor-S gives you the option of simply acquiring and displaying a fully exposed
image, or preserving intermediate exposures.
Acquire
To acquire and display a fully exposed image, click on the Acquire button. An
exposure will be taken based on the time selected in Step III. This is appropriate for
most short exposures.
The progress of the exposure will be displayed in the Exposure Status bar at the
bottom of the acquisition window.
Fig.G-8. Exposure Status bar when acquiring an image.
Depending on which dark subtraction type you have selected, a dark count may be
acquired immediately following image acquisition. See Dark Subtraction Type under
Options, below.
If you want to stop a scan that is in progress, click on the Stop button. The acquisition
will be terminated.