Bio-Rad EXQuest Spot Cutter - Manual (Page 177)

PDQuest User Guide
5-26
Landmarking Gels with Different pH Gradients
If you are combining gels with different pH gradients into the same Master, you will
not be able to landmark the same spot in all the gels. For example, to create a cybergel
from three gels with different pH gradients, you would place landmarks in the
overlapping region of the first two gels, then add the spots from the unique region of
the second gel to the Master. Then you would landmark the overlapping region of the
last two gels.
5.7 Adding Spots to the Master
Recall that all spots initially included in the MatchSet Master come from the template
gel used to create the Master. After you have matched these spots in all the gels, you
are ready to add the unique spots from the other gels to the Master. These may be
spots induced by your experimental protocols, or they may be spots that fall outside
the pH gradient of the current Master.
Auto-Adding Spots
To automatically add the unmatched spots in a MatchSet member to the Master, select
Auto-Add Spots to Master from the Match menu.