Bio-Rad EXQuest Spot Cutter - Manual (Page 147)

PDQuest User Guide
4-26
To cancel all low quantity spots in the set, select Spots > Low Quantity Spots >
Cancel Low Quan. Spots and click in the MatchSet or scanset Gaussian image.
To restore the cancelled low quantity spots, select Spots > Low Quantity Spots >
Restore Low Quan. Spots and click in the MatchSet or scanset Gaussian image.
Quantity Table
You can display the quantity of a spot in each MatchSet gel (or replicate group) using
the Quantity Table function.
Select Quantity Table from the Spots menu, then click on the spot whose quantities in
different gels you want to compare. Alternatively, position the cursor on the spot and
click Shift+F6. A pop-up box will display the quantities of that spot in table format.
Fig. 4-16. Quantity table.
If Replicate Group Quantitation is turned on the average quantity of that spot in each
replicate group will be displayed, as will the coefficient of variance (CV) and the
number of gels in the replicate group (Count).
4.11 Spot Quality
Spot quality is a number ranging from 0 to 100 that is calculated based on five
attributes of each spot: