Pdquest user guide, Choosing your own settings – Bio-Rad EXQuest Spot Cutter User Manual
Page 421
PDQuest User Guide
E-6
Choosing Your Own Settings
If you know the filter and light source settings you want, or want to experiment with
different settings, you can choose them yourself.
Fig. E-5. GS-710 Custom Application controls.
Next to Filter, click on either the Red, Green, Blue, or White checkbox, or a
combination of two of the first three (Red-Green, Green-Blue, Red-Blue).
Fig. E-6. Examples of filter colors and applications.
Next to Light, select the appropriate light source.
•
Reflective mode is for scanning opaque mediums such as dried gels on filter
paper, arrays, or photographs. The scanning dimensions in this mode are 21 cm
x
29 cm.
•
Transmissive mode is for scanning transparent mediums such as films, gels, or
slides. The scanning dimensions in this mode are 20 cm
x
23 cm.
Filter Color
Wavelength
Application Examples
Red
595–750 nm
Coomassie G-250, BCIP/NBT, Fast Green
FCF, Methylene Blue
Green
520–570 nm
Coomassie R-250, Basic Fuchsin
Blue
400–530 nm
Crocein Scarlet
Gray Scale
400–750 nm
Silver Stains, Copper Stains, Film