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Pdquest user guide, Choosing your own settings – Bio-Rad EXQuest Spot Cutter User Manual

Page 437

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PDQuest User Guide

F-6

Choosing Your Own Settings

If you know the filter and light source settings you want, or want to experiment with
different settings, you can choose them yourself.

Fig. F-5. GS-800 Custom Application controls.

Next to Filter, click on either the Red, Green, Blue, or White checkbox, or a
combination of two of the first three (Red-Green, Green-Blue, Red-Blue).

Fig. F-6. Examples of filter colors and applications.

Next to Light, select the appropriate light source.

Select Reflective for scanning opaque mediums such as dried gels on filter paper,
arrays, or photographs. The scanning dimensions in this mode are 30 cm

x

40

cm.

Select Transmissive for scanning transparent mediums such as films, gels, or
slides. The scanning dimensions in this mode are 29 cm

x

40 cm.

Filter Color

Wavelength

Application Examples

Red

595–750 nm

Coomassie G-250, BCIP/NBT, Fast Green

FCF, Methylene Blue

Green

520–570 nm

Coomassie R-250, Basic Fuchsin

Blue

400–530 nm

Crocein Scarlet

White

400–750 nm

Silver Stains, Copper Stains, Film

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