Pdquest user guide, Choosing your own settings – Bio-Rad EXQuest Spot Cutter User Manual
Page 437
PDQuest User Guide
F-6
Choosing Your Own Settings
If you know the filter and light source settings you want, or want to experiment with
different settings, you can choose them yourself.
Fig. F-5. GS-800 Custom Application controls.
Next to Filter, click on either the Red, Green, Blue, or White checkbox, or a
combination of two of the first three (Red-Green, Green-Blue, Red-Blue).
Fig. F-6. Examples of filter colors and applications.
Next to Light, select the appropriate light source.
•
Select Reflective for scanning opaque mediums such as dried gels on filter paper,
arrays, or photographs. The scanning dimensions in this mode are 30 cm
x
40
cm.
•
Select Transmissive for scanning transparent mediums such as films, gels, or
slides. The scanning dimensions in this mode are 29 cm
x
40 cm.
Filter Color
Wavelength
Application Examples
Red
595–750 nm
Coomassie G-250, BCIP/NBT, Fast Green
FCF, Methylene Blue
Green
520–570 nm
Coomassie R-250, Basic Fuchsin
Blue
400–530 nm
Crocein Scarlet
White
400–750 nm
Silver Stains, Copper Stains, Film