Bio-Rad EXQuest Spot Cutter - Manual (Page 185)

PDQuest User Guide
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Manual matches and landmarks will not be removed. Because this command is
irreversible, you will be prompted to complete the operation.
To remove all matches (automatic and manual), as well as landmarks, from a member
image, select Remove All Matches from the Match > Edit Matches submenu and
click on the member image. Because this command is irreversible, you will be
prompted to complete the operation.
5.8.b Higher Level MatchSets
PDQuest allows you to create MatchSets from MatchSets, so you can build a database
by comparing and summarizing the results of a series of experiments.
Higher level MatchSets are MatchSets created from the Masters of lower level
MatchSets. A lower level MatchSet might be a MatchSet created from your original
gels (a level 1 MatchSet), or it could be another higher level MatchSet. Many
experimental protocols can be designed to take advantage of higher level matching.
For example, one experiment may find all the proteins that change during cell growth
in culture, while another may identify nuclear proteins by cell fractionation. These
two experiments can be analyzed individually in level 1 MatchSets, each with their
own Masters. These two Masters could then be combined into a higher level
MatchSet with its own Master. Data from the two experiments can now be compared,
and the higher level MatchSet might shed light on growth-related proteins found in
the nucleus.
Higher level MatchSets are actively linked to the MatchSets used to create them. If
you edit spots in the member images of a level 1 MatchSet, those changes will take
immediate effect in the higher level MatchSet.
For example, when working with a higher level MatchSet, you may notice that a spot
is present in one member and not the other. You could open the level 1 MatchSet with
the missing spot and see if that spot in present in any of the gels. If it is, you could add
it to the Master of the level 1 MatchSet and it would appear in the corresponding
member in the higher level MatchSet, ready to be matched.