Bio-Rad EXQuest Spot Cutter - Manual (Page 515)

PDQuest User Guide
K-8
desired setting. See Table 1, “Recommended Exposure Times and Lenses,” on
page 9.
After you have positioned your sample, click on the Focus button and look at the
image in the acquisition window while adjusting the focus on the camera lens. While
focusing, the camera will limit its focus to a small portion of the sample (this will not
affect any zoom lens adjustments you may have made.) (See the VersaDoc User
Manual for details on focusing.)
When you click on Focus, the light inside the camera box automatically switches on.
To turn the light off while positioning, deselect the Light On checkbox.
When you are finished focusing, click on the Stop button.
K.4 Step III. Set Exposure Time
The “exposure time” is the period of time an image is integrated on the CCD. The
effect is analogous to exposing photographic film to light.
Setting an Exposure Time
Different applications have different optimal exposure times. If you are imaging using
multiple channels, you can select a different exposure time for each channel. First
select the channel using the tabs, then select the appropriate exposure time for that
channel.
See Table 1, “Recommended Exposure Times and Lenses,” on page 9.
You can enter an exposure time (in seconds) directly in the field, or use the Arrow
buttons to adjust the exposure time in 10 percent increments.
Fig.K-5. Selecting an exposure time.