Bio-Rad EXQuest Spot Cutter - Manual (Page 342)

Graphs and Reports
11-7
Column Graphs
Column Graph on the Reports menu and toolbar will fill the right and left margins
with histograms of spots in the Master gel. Select Column Graph; the spots whose
graphs are displayed are all located in a narrow column of the image, centered around
the location of the cursor. The Column Graph only applies to the MatchSet Master. To
change the column of spots, use the Graph Advance/Graph Retreat tools (see below).
Graph Partial Matches
Graph Partial Matches (Match > Edit Matches submenu) displays histograms of each
partially matched spot in the Master. Select the command from the submenu and click
in the Master. Use Graph Advance/Graph Retreat to graph different regions of spots.
Graph Erratic Responses
Graph Erratic Responses (Match > Edit Matches submenu) displays histograms of
each erratic spot in the Master. Select the command from the submenu, click in the
Master, enter a fold change factor in the pop-up box, and click Apply to display the
graphs. Use Graph Advance/Graph Retreat to graph different regions of spots.
Graph Advance/Graph Retreat
If you are displaying Margin Graphs, Column Graphs or graphing spots using the
Graph Partial Matches or Graph Analysis Set commands, you will need to shift the
display to graph spots in different regions of the image. The commands for doing this
are located on the Reports menu or the Graph toolbar.
Graph Advance ( SFT +P AGE D OWN ) shifts the column of spots being graphed to the
right. Graph Retreat ( SFT +P AGE UP ) shifts the column of spots being graphed to the
left. For Margin Graphs, these commands flip through the graph pages.
11.3 A-B Overlay
The A-B Overlay function is used on MatchSet Masters to highlight and compare the
spots that appear in two different MatchSet members.