Bio-Rad EXQuest Spot Cutter - Manual (Page 521)

PDQuest User Guide
K-14
default base file name, the time stamp may change in the course of the series of
exposures; in this case, the base file name will change and the version numbering will
reset for subsequent exposures.)
The highest version number will be the final exposure. If you did not elect to auto-
save the exposures as they were created, then each image will be unsaved.
To stop the image acquisitions, click on the Stop button.
Note: Exposures captured before stopping will be displayed in image windows.
Study the different images and select the best exposure(s) to keep.
K.6 Options
Click on the Options button to open the Options dialog box.
Fig.K-8. Options dialog box.
K.6.a. Dark Subtraction Type
All CCD cameras accumulate electrons that produce a signal that is indistinguishable
from light. This “dark current” adds to the noise in your images, particularly for long