Bio-Rad Firmware & Software Updates - Manual (Page 81)

Data Analysis Windows
70
Standard Curve Chart
The software creates a Standard Curve chart (Figure 50) in the Quantification tab if the data
include sample types defined as standard (Std) for one fluorophore in the run.
Figure 50. Standard Curve chart.
The Standard Curve chart displays the following information:
• Name for each curve (the fluorophore or target)
• Color of each fluorophore or target
• Reaction efficiency (E). Use this statistic to optimize a multiplex reaction and to
equalize the data for a standard curve
NOTE: The reaction efficiency describes how much of your target is being
produced with each cycle in the protocol. An efficiency of 100% means that you
are doubling your target with each cycle.
• Coefficient of determination, R 2 (written as R^2). Use this statistic to determine how
correctly the line describes the data (goodness of fit)
• Slope
• y-intercept
Chart Right-Click Menu Options
In addition to the common right-click menu options to copy, print and export charts, Table
lists the menu options available only on the Amplification chart.
Table 23. Right-click menu items for spreadsheets.
Menu Option Function
Well XX, Fluor/Target View only this well, remove this well from view, set color for
this trace, or exclude this well from analysis.
Selected Traces View only these wells, remove these wells from view, set
color for these traces, or exclude these wells from analysis.
Show Threshold Values Display the threshold value for each amplification curve on
the chart.
Trace Styles... Open the Trace Styles window to change trace styles that
appear on the Quantification and Melt Curve tabs.