7 data analysis windows, Quantification tab, Chapter 7. data analysis windows – Bio-Rad Firmware & Software Updates User Manual
Page 78: Data analysis windows
MiniOpticon Instruction Manual
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7
Data Analysis Windows
Read this chapter for more information about the tabs in the Data Analysis window:
• Quantification tab (page 67)
• Quantification Data tab (page 71)
• Melt Curve Data tab (page 75)
• Allelic Discrimination tab (page 79)
• Custom Data View tab (page 81)
• Run Information tab (page 83)
• Well Groups Reports (page 87)
NOTE: The tabs displayed in the data analysis window can be customized by
selecting them in the View menu. This layout will be saved with the file.
Quantification Tab
Use the data in the Quantification tab (Figure 47) to set the data analysis conditions, including
the baseline settings for individual wells and the threshold settings. The Quantification tab
shows data in these four views:
• Amplification chart. Shows the relative fluorescence units (RFUs) for each well at every
cycle. Each trace in the chart represents data from a single fluorophore in one well
• Standard curve. This graph is only shown if the run includes wells designated as
Sample Type Standard. It shows a standard curve with the threshold cycle plotted
against the log of the starting quantity. The legend shows the Reaction Efficiency (E) for
each fluorophore in the wells with a standard sample type
• Well selector. Selects the wells with the fluorescence data you want to show