Bio-Rad Firmware & Software Updates - Manual (Page 144)

MiniOpticon Instruction Manual
133
Right-click for graph , 94
Scaling options , 93
Spreadsheet , 94
Gene Study , 101
Inter-run calibration , 101
Preparing data , 103
Report , 104
Show Details , 104
Study Analysis tab , 102, 104
Study Setup tab , 103
GOI
Normalization factor , 107, 108
Normalized expression , 108, 109, 110
Relative quantity , 106, 107
SD Normalized expression , 110
GOTO
Adding repeats , 26
Inserting , 34
Gradient
Inserting , 34
Step , 34
Gradient Calculator , 32
H
Heated plate , 2
I
Import Template , 51
Increment , 36
Inner lid , 2
Instruments
Safety , iii
Warning labels , iii
Inter-run calibration , 101
L
Layout
Quantitation tab , 56, 68
LED on lid , 2
Library , 45
Lid
Green LED , 2
Opening , 26
M
Magnification in Plate Editor , 41
Master Mix Calculator , 15
Melt Curve
Adjusting data , 75
Data analysis , 74
Inserting , 35
Opening tab , 74
RFU data , 74
Melt Curve Data , 76
Amplification Data (RFU) , 77
Plate , 76
Tab , 75
Melt Peaks
Spreadsheet , 76
Melt Peaks Spreadsheet , 76
Menu bar
Data analysis , 55
Main software , 10
Plate Editor , 40
Protocol Editor , 32
Mode
Baseline Subtracted , 59
Baseline Subtracted Curve Fit , 59
Not Baseline Subtracted , 59
Temperature control , 36
Multiple instruments
Viewing , 14
M-value , 93
N
Normalization factor
Relative quantity , 107, 108
Normalized expression
Formula , 108
Formula for GOI , 111
Scaled to highest , 109
Scaled to lowest , 109, 110
Standard deviation (SD) , 110
Standard deviation formula , 109
Normalized GOI formula , 111
Normalizing
Gene expression , 91
Not Baseline Subtracted mode , 59
Notes
Well , 45
O
Open Lid button , 26