Bio-Rad Firmware & Software Updates - Manual (Page 137)

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Calibration Wizard
The MiniOpticon system is factory calibrated for commonly used fluorophores in Bio-Rad
white-welled plates (Table 43).
To open the Calibration Wizard to calibrate the MiniOpticon system:
1. Select an instrument in the Detected Instruments pane.
2. Select Tools > Dye Calibration Wizard to open the Dye Calibration window and
calibrate new dye and plate combinations (Figure 85).
Figure 85 shows an example of the Dye Calibration window.
Figure 85. Dye Calibration window.
Calibrating the MiniOpticon System
To calibrate the MiniOpticon system in the Dye Calibration window:
1. Select the Plate Type in the Calibrate New or Existing Fluorophores pane. If the plate
type is not included in the list, type the name in the box to add it to the list.
2. Select the fluorophore you want to calibrate from the pull-down list. If the fluorophore
name is not included in the list, type the name in the box to add it to the list.
3. Begin preparing a 48-well plate for dye calibration by pipetting dye solution into each
well. For each fluorophore, fill each well with 50 µl of 300 nM dye solution.
4. Seal the plate using the sealing method you will use in your experiment.
5. Click Next and follow the on-screen instructions. First, place an empty plate in the
MiniOpticon system for the first procedure of the calibration. Next, place the calibration
Table 43. Factory calibrated fluorophores, channels, and instruments.
Fluorophores Channel
FAM, SYBR
®
Green I
1
HEX 2