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Experiment settings window, Show details option – Bio-Rad Firmware & Software Updates User Manual

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MiniOpticon Instruction Manual

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Show Details Option

When Show Details is selected from the right-click menu of the Gene Expression spreadsheet,
the spreadsheet shows the information listed in Table 37.

Experiment Settings Window

Open the Experiment Settings window by clicking the Experiment Settings button in the
Gene Expression tab. In this window, view or change the list of Targets and Samples, select
reference genes, select control samples or set the Gene Expression Analysis sample group to
be analyzed if Biological Set Names have been added to the wells (Figure 67).

Figure 67. Experiment Settings window with Targets tab selected.

To adjust the lists in these tabs, use the following functions:

Table 37. Information in the Gene Expression spreadsheet with Show Details selected.

Information

Description

Data Set

Fluorescence data from one fluorophore in the data file

Relative Quantity

Calculated relative quantity of samples

Relative Quantity SD

Standard deviation of the relative quantity calculation

Corrected Relative Quantity
SD

Calculated standard deviation of the corrected relative
quantity

Relative Quantity SEM

Standard error of the mean of the relative quantity calculation

Corrected Relative Quantity
SEM

Calculated standard error of the mean of the corrected relative
quantity

Unscaled Expression

Calculated unscaled expression

Unscaled Expression SD

Calculated Standard Deviation unscaled expression

Corrected Unscaled
Expression SD

Calculated Standard Deviation of the unscaled expression

Unscaled Expression SEM

Calculated Standard Error of the Mean unscaled expression

Corrected Unscaled
Expression SEM

Calculated Standard Error of the Mean of the unscaled
expression

Expression

Relative expression level

Wells

Well number in the plate