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Insert melt curve button, Step options – Bio-Rad Firmware & Software Updates User Manual

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MiniOpticon Instruction Manual

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2. Edit the GOTO step number or number of GOTO repeats by clicking the default number

in the graphic or text view, and entering a new value.

Figure 24 shows an inserted GOTO step at the end of the protocol. Notice that the GOTO loop
includes steps 2 through 4.

Insert Melt Curve Button

To insert a melt curve step before or after the selected step:

1. Click the Insert Melt Curve button.

2. Edit the melt temperature range or increment time by clicking the default number in the

graphic or text view, and entering a new value. Alternatively, click the Step Options
button to enter the gradient range in the Step Options window (page 36).

NOTE: You cannot insert a melt curve step inside a GOTO loop.

NOTE: The melt curve step includes a 30 second hold at the beginning of the step
that is not shown in the protocol.

Figure 25 shows a melt curve step added after step 6:

Figure 25. Protocol with inserted melt curve step.

Step Options

To change a step option for the selected step:

1. Select a step by clicking on the step in the graphic or text view.

2. Click the Step Options button to open the Step Options window.

3. Add or remove options by entering a number, editing a number, or clicking a check box.

TIP: To hold a step forever (an infinite hold), enter zero (0.00) for the time.