Bio-Rad Firmware & Software Updates - Manual (Page 45)

Protocols
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2. Click the Add Plate Read to Step button to add a plate read to the selected step. If the
step already contains a plate read, the text on the button changes, so now the same
button reads Remove Plate Read. Click to remove a plate read from the selected step.
Insert Gradient Button
To insert a gradient step before or after the currently selected step:
1. Insert a temperature gradient step by clicking the Insert Gradient button.
2. Make sure the plate size for the gradient matches the block type of the instrument.
Select the plate size for the gradient by selecting Tools > Gradient Calculator in the
Protocol Editor menu bar.
3. Edit the gradient temperature range by clicking the default temperature in the graphic or
text view, and entering a new temperature. Alternatively, click the Step Options button
to enter the gradient range in the Step Options window (page 36)
4. Edit the hold time by clicking the default time in the graphic or text view, and entering a
new time.
Figure 24 shows the inserted gradient step. The temperatures of each row in the gradient are
charted on the right side of the window.
Figure 24. Protocol with inserted gradient step.
Insert GOTO Button
To insert a GOTO step before or after the selected step:
1. Click the Insert GOTO button.