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Protocol editor controls, Insert step button, Add or remove a plate read – Bio-Rad Firmware & Software Updates User Manual

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MiniOpticon Instruction Manual

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Table 13 lists the function of the Protocol Editor toolbar buttons:

Protocol Editor Controls

The Protocol Editor window includes buttons for editing the protocol. First, select and highlight
a step in the protocol by left clicking it with the mouse. Then click one of the Protocol Editor
buttons at the bottom left side of the Protocol Editor window to change the protocol. The
location for inserting a new step, “Before” or “After” the currently selected step is determined
by the status of the Insert Step box located in the toolbar.

Insert Step Button

To insert a temperature step before or after the currently selected step:

1. Click the Insert Step button.

2. Edit the temperature or hold time by clicking the default value in the graphic or text view,

and entering a new value.

3. (Optional) Click the Step Options button to enter an increment or extend option to the

step (page 36).

Add or Remove a Plate Read

To add a plate read to a step or to remove a plate read from a step:

1. Select the step by clicking the step in either the graphical or text view.

Table 13. Protocol Editor toolbar buttons

Toolbar Button and Menus

Name

Function

Save

Save the current protocol file.

Print

Print the selected window.

Insert Step

Select After or Before to insert steps in a
position relative to the currently highlighted
step.

Sample Volume

Enter a sample volume in µl between 0 and
50. If you are using higher than 50 µl
reactions, select 50 µl.
Sample volume determines the Temperature
Control mode. Enter zero (0) to select Block
mode.

Est. Run Time

View an estimated run time based on the
protocol steps and ramp rate.

Help

Open the software Help for more
information about protocols.