Bio-Rad Firmware & Software Updates - Manual (Page 54)

MiniOpticon Instruction Manual
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Figure 29. Auto layout tab of Setup Wizard.
Create a plate layout by following these steps:
1. Auto layout tab. Select the area in which samples will be located on the plate by clicking
on a well at one edge and then dragging to encompass the area required. Enter the
number of targets and samples to be loaded. If the numbers entered do not fit in the area
selected, you will need to reduce the numbers or increase the plate area. The orientation
of items on the plate and their grouping can be specified.
NOTE: If your plate layout does not have a regular pattern, use the Target names
tab to manually position your targets anywhere on the plate. Click and drag to
select mulitple wells.
2. Target names tab. Click a target grouping and then enter its name in the box provided.
Multiple target groups can be selected by clicking and dragging.
TIP: After entering a name, press Tab to advance to the next grouping on the right
or Enter to move to the next group below.
3. Sample names tab. Click a sample grouping and then enter its name in the box
provided. Multiple sample groups can be selected by clicking and dragging.
TIP: When entering target or sample names, click and hold the control key to select
groups of wells that are not adjacent.
4. Reference targets tab. Click on one or more targets to use as references for normalized
gene expression.
5. Control sample tab. Click on one sample to use as a control for relative gene
expression calculations.