Bio-Rad Firmware & Software Updates - Manual (Page 112)

MiniOpticon Instruction Manual
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Right-click on the heat map to select the items shown in Table 38.
Data Spreadsheet
The spreadsheet lists the target, sample, and regulation. Select chart options by right-clicking.
Results
The Results spreadsheet summarizes the data from all of the charts. The contents of the
Results spreadsheet are listed in Table 40.
Table 40. Information in the Results tab.
NOTE: Data for replicates are found only in the spreadsheets of data analysis tabs
in which Split Out Replicates has been selected (i.e. Clustergram and Heat Map).
Gene Study
Create a Gene Study to compare gene expression data from one or more real-time PCR
experiments using an inter-run calibrator to normalize between the experiments. Create a
Gene Study by adding data from one or more data files (.pcrd extension) to the Gene Study,
the software groups them into a single file (.mgxd extension).
NOTE: The maximum number of samples you can analyze in a Gene Study is
limited by the size of the computer's RAM and virtual memory.
Gene Study Inter-Run Calibration
Inter-run calibration is automatically attempted in every gene study for each target to
normalize inter-run variations between targets assayed in separate real-time PCR runs (i.e
different .pcrd files).
Information Description
Target Target Name (amplified gene)
Sample Sample Name
Mean Cq Mean of the quantification cycle
Mean Efficiency Corrected Cq Mean of the quantification cycle after adjusting for the reaction
efficiency
Normalized Expression Target expression normalized to a reference target (C
q
)
Relative Normalized
Expression Normalized expression relative to a control sample. Also called
Fold Change
Regulation Change in expression relative to a control sample
Compared to Regulation
Threshold Up- or downregulation of an experimental sample based on
the threshold setting
P-Value The probability that a difference in expression is significant
Exceeds P-Value Threshold Indication of whether or not the p-value for a target exceeds a
threshold