Bio-Rad Firmware & Software Updates - Manual (Page 48)

MiniOpticon Instruction Manual
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• Calculated mode. When you enter a sample volume between 1 and 50 µl the thermal
cycler calculates the sample temperature based on the sample volume. This is the
standard mode
• Block mode. When you enter a sample volume of zero (0) µl, the thermal cycler records
the sample temperature as the same as the measured block temperature
Protocol AutoWriter
Open the Protocol AutoWriter to quickly write protocols for PCR and real-time PCR runs. To
open the Protocol AutoWriter, select one of these options:
• Click the Protocol AutoWriter button in the main software window toolbar
• Select Tools > Protocol AutoWriter from the menu bar in the main software window
Figure 27 shows a protocol (bottom of window) written by the Protocol AutoWriter.
Figure 27. Protocol AutoWriter window with a new protocol.
Creating a Protocol with the Protocol AutoWriter
Follow these steps to use the Protocol AutoWriter to create a new protocol:
1. Click the Protocol AutoWriter button on the toolbar to open the Protocol AutoWriter
window.
2. Enter the Annealing Temperature (Ta) and Amplicon Length in the boxes within the
Enter Target Values/Enzymes pane. If you do not know the annealing temperature for
primers, click the Ta Calculator button to enter the primer sequences and calculate the
annealing temperature. For information about the calculations used in the Ta Calculator
see Breslauer et al. 1986.