Bio-Rad Firmware & Software Updates - Manual (Page 123)

Gene Expression Analysis
112
Where
• n = Number of reference targets (genes)
• SD = Standard deviation
The standard error for the normalization factor in the normalized expression formula is shown
here:
Where:
• n = Number of reference targets
• SE = Standard error
• NF = Normalized expression
• RQ = Relative quantity
The standard error for normalized gene of interest (GOI) formula is shown here:
Where:
• SE = Standard error
• GOI = Gene of interest (one target)
• NF = Normalization factor
• n = Number of reference targets
SE NF
n
NF
n
SE RQ
sample (Ref 1)
n SE RQ
sample (Ref 1)

---------------------------------------------------------  
  2 SE RQ
sample (Ref 2)
n SE RQ
sample (Ref 2)

---------------------------------------------------------  
  2

SE RQ
sample (Ref n)
n SE RQ
sample (Ref n)

---------------------------------------------------------  
  2
+ + +

=
SE GOI
n
GOI
n
SE NF
n
NF
n
----------------  
  2 SE GOI
GOI
-----------------  
 
+
2

=