Bio-Rad Firmware & Software Updates - Manual (Page 34)

MiniOpticon Instruction Manual
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• File > Open > PrimePCR Run File... from the main window
Once a PrimePCR run has been selected, the Run Setup window will open on the Start Run
tab with the default PrimePCR protocol and plate layout loaded based on the instrument
selected.
To reduce the overall run time, the melt step can be removed by unchecking the box adjacent
to Include Melt Step on the Protocol tab. Any other modifications to a PrimePCR run
protocol are not recommended since the default protocol was used for assay validation and
any deviation from this may affect the results. Changes that are made will be noted in the Run
Information tab of the resultant data file and in any reports that are created.
To import target information for PrimePCR plates into a run’s plate layout, select Plate Setup >
Apply PrimePCR File from the Real-time Status tab (page 27) or from the Data Analysis
window (page 53) and choose the appropriate file (.csv). Select this file by searching in the
PrimePCR folder using part of the file name or by browsing to the location on the computer
where the file was downloaded from the Bio-Rad website when the plate was ordered.
Protocol Tab
The Protocol tab shows a preview of the selected protocol file loaded in the Run Setup
(Figure 17). A protocol file contains the instructions for the instrument temperature steps, as
well as instrument options that control the ramp rate and lid temperature.
Select one of the following options to select an existing protocol, create a new protocol, or edit
the currently selected protocol:
• Create New button. Open the Protocol Editor to create a new protocol
• Select Existing button. Open a browser window to select and load an existing protocol
file (.prcl extension) into the Protocol tab
• Express Load pull-down menu. Quickly select a protocol to load it into the Protocol tab
TIP: To add or delete protocols in the Express Load menu, add or delete files (.prcl
extension) in the ExpressLoad folder. To locate this folder, select Tools > User
Data Folder in the menu bar of the main software window
• Edit Selected button. Open the currently selected protocol in the Protocol Editor
End Point Only Runs
To run a protocol that contains only an end point data acquisition step, select Options > End
Point Only Run from Options in the menu bar of the Run Setup window. The default end point
protocol, which includes two cycles of 60.0°C for 30 seconds, is loaded into the Protocol tab.
To change the step temperature or sample volume for the end point only run, click the Start
Run tab and edit the Step Temperature or Sample Volume.
Plate Tab
The Plate tab shows a preview of the selected plate file loaded in the Run Setup (Figure 18). In
a real-time PCR run, the plate file contains a description of the contents of each well, the scan
mode, and the plate type. CFX Manager software uses these descriptions for data collection
and analysis.
Select one of the following options to select an existing plate, create a new plate, or edit the
currently selected plate: