Bio-Rad ChromLab™ Software - Manual (Page 62)

3 | Setting Up NGC Chromatography Systems
60 | NGC Chromatography Systems and ChromLab Software
Connecting Tubing to the Columns
Note: Ensure that the system has been purged of air and that the tubing is
filled with buffer before connecting the columns.
To connect tubing to the columns
1. Connect tubing from one of the following modules to the top of the column:
 Sample inject valve column port
 Column switching valve inlet port (1–5)
Note: For best air-free connection ensure that the tubing is dripping buffer
at a flow rate of 0.5–1 ml/min. Fill the top of the column with buffer before
inserting and tightening the fitting.
2. Remove the plug from the bottom of the column.
3. Connect the tubing from one of the following modules to the bottom of the
column:
 UV monitor
 Column switching valve outlet port (1–5)