Bio-Rad ChromLab™ Software - Manual (Page 130)

5 | Plumbing and Priming the NGC Chromatography Systems
128 | NGC Chromatography Systems and ChromLab Software
5. Select Q4 as the port to open and prime.
6. Insert a 30 ml syringe into the priming port on system pump A.
7. Turn the priming port valve counter-clockwise one full turn to open the seal.
8. Slowly withdraw the plunger to withdraw liquid from the port until air no
longer enters the syringe (approximately 10 ml) and the tubing is filled with
liquid.
9. Turn the priming port valve clockwise to close the port.
10. Remove the syringe from the priming port and discard the liquid into waste.
11. Repeat steps 6–10 for ports Q3–Q1.
12. To prime system pump B, select port Q3 and perform steps 6–10.