Bio-Rad ChromLab™ Software - Manual (Page 126)

5 | Plumbing and Priming the NGC Chromatography Systems
124 | NGC Chromatography Systems and ChromLab Software
Priming and Purging the NGC Quest System
Caution: Before priming or purging the system, ensure that any attached
columns are offline. The priming process occurs at a high flow rate and can
significantly harm or destroy attached columns.
Priming and purging the NGC Quest system requires access to ChromLab. You can
perform the steps on the NGC touch screen.
Important: T he instrument’s buffer lines cannot be primed when the system is
under pressure. Before opening the priming port, turn the inject valve to Waste
to release the system pressure.
To prime the system pumps
1. Immerse the inlet tubing for both system pumps A and B in a container of
buffer or HPLC grade (filtered, degassed) or other high-quality water.
2. Insert a 30 ml syringe into the priming port on system pump A.
3. Turn the priming port valve counter-clockwise one full turn to open the seal.
4. Slowly withdraw the plunger to withdraw liquid from the port until air no
longer enters the syringe (approximately 10 ml) and all tubing is filled with
liquid.
5. Turn the priming port valve clockwise to close the port.