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Bio-Rad ChromLab™ Software User Manual

Page 110

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5

| Plumbing and Priming the NGC

Chromatography

Systems

108

| NGC Chromatography Systems and ChromLab Software

To plumb the system pumps

1.

Connect inlet tubing to the inlet manifold of system pump A.

2.

Connect inlet tubing to the inlet manifold of system pump B.

3.

Immerse the open ends of both inlet tubing in a bottle of degassed, deionized
water.

To plumb the fraction collector

1.

Connect Tube #4 to the open end of the backpressure regulator that you
connected to the conductivity monitor in step

2 on page

105.

2.

Connect the other end of Tube #4 to the Common port on the BioFrac fraction
collector diverter valve.