Bio-Rad ChromLab™ Software - Manual (Page 134)

5 | Plumbing and Priming the NGC Chromatography Systems
132 | NGC Chromatography Systems and ChromLab Software
4. In the fluidic scheme, touch or click the System Pump module to open its
dialog box.
5. In the System Pump dialog box, select Priming on the Mode dropdown list.
Note: When in Priming mode, the sample inject valve switches the system
pump flow to waste (W2). After the process is completed, the sample inject
valve switches the system pump to manually load the column through the
loop.
6. Select Q4 as the port to open and prime.