Priming and purging the ngc scout system – Bio-Rad ChromLab™ Software User Manual
Page 129
Priming and Purging the System
Installation Guide
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Priming and Purging the NGC Scout System
Priming the NGC Scout system is a manual procedure and involves priming the
buffer blending valve starting from the Q4 port to Q1. Purging the NGC Scout
system is an automated procedure and involves removing air from one or all buffer
lines as well as the system pumps. Both procedures require access to ChromLab.
Tip:
You can perform the steps on the NGC touch screen.
Priming the NGC Scout System
Note:
This section assumes that you have not introduced buffer into the lines.
If you have buffer in the lines and are purging the lines of small air bubbles,
proceed to
Purging the NGC Scout System on page
.
To prime the buffer blending valve
1.
Immerse the inlet tubing for ports Q4–Q1 in their respective buffer containers.
(See
To plumb the buffer blending valve on page
for more information.)
2.
Set the fluidic scheme to either NGC Scout or NGC Scout Plus.
3.
In the fluidic scheme, touch or click the System Pump module to open its
dialog box.
4.
In the System Pump dialog box, select Priming in the Mode dropdown list.
By default, all ports are closed. The Closed radio button is selected.