Bio-Rad ChromLab™ Software - Manual (Page 136)

5 | Plumbing and Priming the NGC Chromatography Systems
134 | NGC Chromatography Systems and ChromLab Software
To prime the buffer inlet valves
1. Immerse the tubing for the inlet ports in their respective buffer containers.
2. On the NGC touch screen, open the System Pump dialog box.
3. In the System Pump dialog box, select the first inlet port to prime, for example
Buffer A 1.
4. Insert a 30 ml syringe into the priming port on system pump A.
5. Turn the priming port valve counter-clockwise one full turn to open the seal.
6. Slowly withdraw the plunger to withdraw liquid from the port until air no
longer enters the syringe (approximately 10 ml) and all the tubing is filled
with liquid.
7. Turn the priming port valve clockwise to close the port.
8. Remove the syringe from the priming port and discard the liquid into waste.