Bio-Rad ChromLab™ Software - Manual (Page 142)

5 | Plumbing and Priming the NGC Chromatography Systems
140 | NGC Chromatography Systems and ChromLab Software
To purge the buffer inlet valves
1. Open the System Pump dialog box and select Priming on the Mode dropdown
list.
Note: When in Priming mode, the sample inject valve switches the system
pump flow to waste (W2). After the process is completed, the sample inject
valve switches the system pump to manually load the column through the
loop.
2. Select the first buffer port to purge, for example Buffer A 1.
3. (Optional) Change the default flow rate.
The default flow rate is set to 10 ml/min.
4. Click Start.
5. Repeat steps 2–4 for the remaining buffer inlet ports.