Bio-Rad ChromLab™ Software - Manual (Page 104)

5 | Plumbing and Priming the NGC Chromatography Systems
102 | NGC Chromatography Systems and ChromLab Software
To plumb and prime the system pump wash lines
1. Mount a pump head wash tube holder to the right or left side of the instrument
in either the upper or lower rack slots.
2. Fill a 50 ml tube with 20% isopropanol (IPA) and insert it in the tube holder.
Tip: You can use 20% ethanol as an alternative.
3. Connect inlet tubing to the pump wash inlet port (left rear port) on
system
pump A.
4. Immerse the other end of the pump A wash inlet tubing in the tube of 20% IPA.
5. Connect the pump head wash priming tubing to the outlet (right rear port) on
system pump B.
6. Locate a 1/4-28 female to female luer adaptor in the fittings kit and connect it to
the pump head wash priming tubing.
7. Connect a 1/4-28 union to a second inlet tubing. You will use the inlet tubing as
the outlet line for system pump B.
Tip: Set the inlet tubing with the attached union aside. Keep it close by, as
you will attach it to the pump head wash tubing in step 11.