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Priming and purging the system – Bio-Rad ChromLab™ Software User Manual

Page 125

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Priming and Purging the System

Installation Guide

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Priming and Purging the System

This section explains how to prime your system before running the pumps, and

how to purge your system of air bubbles or rapidly exchanging buffer

solutions.

Note:

This section assumes that you primed the wash system when you

plumbed the system. See

Plumbing All NGC Systems on page

100

for more

information.

Priming and purging tasks are specific to the NGC system. Table

13 lists the tasks

associated with each system configuration. The tasks are explained in detail in the

sections that follow.

Prime and purge the
inlet port on system
pump A

Prime and purge Q1–Q4
on the buffer blending
valve

Prime and purge Q1–Q4 on
the buffer blending valve

Prime and purge the
inlet port on system
pump B

Prime and purge the buffer
inlet valves A and B

Prime and purge the
sample pump

Table 13.

Priming and purging tasks for each NGC system configuration

NGC Quest

NGC Scout

NGC Discover

Tip:

The inlet ports

on system pumps A
and B are primed
and purged when
the buffer blending
valve is primed.

Tip:

The inlet ports on

system pumps A and B
are primed and purged
when the buffer
blending valve is
primed.