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Calibrating the pump flow rate, Calibrating pressure sensor zero point – Bio-Rad ChromLab™ Software User Manual

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Calibrating the Instrument

Installation Guide

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Calibrating the Pump Flow Rate

Prime and purge the system pumps with water before starting the calibration

procedure. Calibrate the flow rate with water.

Note:

Ensure that the column is offline before performing this task.

To calibrate the pump flow rate

1.

On the NGC touch screen, select Calibrate on the ChromLab dropdown menu.

2.

Select Pump Flow Rate on the dropdown list.

3.

Follow the steps in the Pump Flow Rate Calibration dialog box.

4.

Verify that Status (at the bottom of the dialog box) is Calibrated.

Calibrating Pressure Sensor Zero Point

Use this procedure to zero the pressure on the following modules:

Sample pump

System pump

Column switching valve

To zero the pressure

1.

Ensure the modules are not plumbed and are at ambient pressure.

2.

On the NGC touch screen, select Calibrate on the ChromLab dropdown menu.

3.

Select the pump or valve on the dropdown list.

4.

Follow the steps in the Calibration dialog box.

5.

Verify that Status (at the bottom of the dialog box) is Calibrated.