2 glossary – Metrohm 792 Basic IC User Manual

Page 67

4 Operation

792 Basic IC

4.1.2 Glossary

System

The term “system“ is used to describe the combination of

instrument

settings, time program and processing method, which have been

optimized for the specific separating column and the determination to

be carried out with it. A system is used to start single determinations or

determinations with the help of a sample queue.
Systems are stored as

system files (

*.smt

) in the

Systems

directory.

Method

A method contains all information necessary for

data acquisition, in-

tegration, peak evaluation and quantification. It can be considered

as the chromatogram template, i.e. chromatogram without raw data.
Methods are stored as

method files (

*.mtw

) in the

Methods

directory.

Each system is linked to a method. This method is called

processing

method and is opened automatically at the start of a new determina-

tion.

Chromatogram

A chromatogram is a graphic plot of the elution curve (signal vs. time)

recorded following a chromatographic separation on a separating col-

umn.
Chromatograms are stored as

chromatogram files (

*.chw

) in the

Data

directory. As well as the measuring data, the chromatogram files also

contain the method parameters and system settings, which have been

used for data recording, data processing and remote control.

Determination

In order to carry out a determination a suitable

system must be se-

lected for the separating problem. The result of the determination is a

chromatogram, in which the measuring data and results of the deter-

mination are stored.

Calibration

Calibration is used to describe the method of determining the relation-

ship between the peak height or peak area found for one component

and its concentration in the sample. The result of the calibration is a

calibration function (calibration curve), which shows the relationship

between the amount of sample and the evaluated quantity.
The determination of the calibration function with reference solutions

can be carried out as a

one-point or as a multiple-point calibration.

The calibration method that is mainly used in ion chromatography is the

external standard calibration (absolute calibration); calibrations with

internal standard (relative calibration) or tabulated calibrations

are also possible.