7 precolumns and separating columns, 1 general information on precolumns, 2 precolumns with twin cartridge holder – Metrohm 792 Basic IC User Manual
Page 37
2 Installation
792 Basic IC
28
2.7
Precolumns and separating columns
2.7.1
General information on precolumns
The use of easily exchangeable precolumns protects the separating
columns and prolongs their lifetime. The precolumns available from
Metrohm (see section 6.3.2) are either real precolumns or precolumn
cartridges, which are used together with the 6.2821.050 Twin cartridge
holder or the 6.2828.110 Precolumn cartridge holder.
New IC precolumns are normally filled with solution and sealed at both
ends. Before the precolumn is installed in the system, it must be
ensured that this solution is freely miscible with the eluent used
(check manufacturer's specifications).
2.7.2
Precolumns with twin cartridge holder
The 6.1005.020, 6.1005.040, 6.1005.050, 6.1007.010 and 6.1010.010
Precolumn cartridges are installed in the 6.2821.050 Twin cartridge
holder as follows (see Fig. 10):
1 Install cartridge
x Insert inlet capillary 55
55
55
55 with end piece for precolumn cartridge
in Manufit housing 56
56
56
56.
x Insert outlet capillary 61
61
61
61 with end piece for precolumn car-
tridge in Manufit pressure screw 60
60
60
60.
x Remove end caps from the precolumn cartridge 59
59
59
59 (the steel
mesh 58
58
58
58 and gaskets 57
57
57
57 are already inserted in the car-
tridge).
x Fit the two capillary end pieces onto the precolumn cartridge
59
59
59
59 (comply with the flow direction if specified on the column).
x Screw Manufit pressure screw 60
60
60
60 and Manufit housing 56
56
56
56
firmly together.
2 Connect precolumn
x Cut inlet capillary 55
55
55
55 of the assembled precolumn to the
desired length and mount a compression fitting 46
46
46
46 (see sec-
tion 2.5).
x Connect inlet capillary 55
55
55
55 either using the Coupling 26
26
26
26 to the
column connection capillary 20
20
20
20 mounted on the injection
valve or directly to connection "4“ of injection valve A.
x Shorten outlet capillary 61
61
61
61 of the precolumn to ca. 5 cm and
mount a compression fitting 46
46
46
46 (see section 2.5).