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2 glossary – Metrohm 761 Compact IC User Manual

Page 78

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4 Operation

761 Compact IC

68

4.1.2 Glossary

System

The term “system“ is used to describe the combination of instrument

settings, time program and process method which have been opti-

mized for the specific separating column and the determination to be

carried out with it. A system is used to start single determinations or de-

terminations with the help of a sample queue.
Systems are stored as system files (

*.smt

) in the

Systems

directory.

Method

A method contains all information necessary for data acquisition, in-

tegration, peak evaluation and quantification. It can be considered

as the chromatogram template, i.e. chromatogram without raw data.
Methods are stored as method files (

*.mtw

) in the

Methods

directory.

Each system is linked to a method. This method is called processing

method and is opened automatically at the start of a new determina-

tion.

Chromatogram

A chromatogram is a graphic plot of the elution curve (signal vs. time)

recorded following a chromatographic separation on a separating col-

umn.
Chromatograms are stored as chromatogram files (

*.chw

) in the

Data

directory. As well as the measuring data the chromatogram files also

contain the method parameters and system settings which have been

used for data recording, data processing and remote control.

Determination

In order to carry out a determination a suitable system must be se-

lected for the separating problem. The result of the determination is a

chromatogram, in which the measuring data and results of the deter-

mination are stored.

Calibration

Calibration is used to describe the method of determining the relation-

ship between the peak height or peak area found for one component

and its concentration in the sample. The result of the calibration is a

calibration function (calibration curve), which shows the relationship

between the amount of sample and the evaluated quantity.
The determination of the calibration function with reference solutions

can be carried out as a one-point or as a multiple-point calibration.

The calibration method which is mainly used in ion chromatography is

the external standard calibration (absolute calibration); calibration

with an internal standard (relative calibration) or tabulated calibra-

tion are also possible.